L-Aspartate Dehydrogenase (ASPDH)

ASPDH, Belongs to the L-aspartate dehydrogenase family, Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate.A gene encoding an L-aspartate dehydrogenase homologue was identified in the anaerobic hyperthermophilic archaeon Archaeoglobus fulgidus. After expression in Escherichia coli, the gene product was purified to homogeneity, yielding a homodimeric protein with a molecular mass of about 48 kDa. Characterization revealed the enzyme to be a highly thermostable L-aspartate dehydrogenase, showing little loss of activity following incubation for 1 h at up to 80 degrees C. The optimum temperature for L-aspartate dehydrogenation was about 80 degrees C.